A novel Ca2+ indicator for long-term tracking of intracellular calcium flux

A novel Ca2+ indicator for long-term tracking of intracellular calcium flux

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The major drawback of using Fluo-4 AM is that it requires an organic anion transporter inhibitor, such as probenecid, to prevent leakage.This can hinder the studies that require extended monitoring time and longer cellular retention.To address the issue, a Siren novel Ca2+ indicator, Calbryte 520 AM, was developed.We compared the performance of Fluo-4 AM and Calbryte 520 AM following prolonged incubation periods after cell loading.Cells VISION SUPORT II loaded with Calbryte 520 AM retained the dye for up to 24 h while exhibiting significant fluorescence brightness and superior Fmax/F0 ratios (Fmax: fluorescence intensity upon stimulation; F0: intensity before stimulation).

It demonstrated that the longer retention of Calbryte 520 AM can be exploited to accommodate for the extended time required when monitoring calcium dynamics.